alternative SPRI beads

[aerseq]

Is anyone using SPRI beads other than Beckman Coulter's Agencourt AMPure XP? Is anyone using MagBio's HighPrep PCR beads?

[luc]

Yep, we have been using MagBio's HighPrep PCR beads and the KapaPure beads. Both worked similar to the Ampure XP beads, also for size selections.

[melop]

We just follow the DIY SPRI bead protocol here https://openwetware.org/wiki/SPRI_bead_mix. We tested our home-brewed mixture with Ampure beads and didn't find much difference.

It is extremely cheap to make compared to the Ampure beads. You could make a liter of it for 400 bucks.

[sequencingfan]

There is also a solution PCRClean Dx. Did anyone try this one?

[Simone78]

we use SeraMag beads (carboxyl beads, hydrophylic). With a 150 EUR bottle (15 ml) one can make 750 ml of bead solution. We follow the protocol detailed in this paper: PMID:22267522

[NGSMicro]

Also using homemade beads with the same protocol as melop, much cheaper.

If you're worried they aren't as good you can validate them against whatever you're using now.

[kwaraska]

PCR Clean DX

These are from Aline Biosciences.

In my last lab our robotic company tried to tell me our preps weren't working because we used Aline. They sent us Ampure beads and we did a side-by-side comparison. Aline performed better and at about 1/3 the cost.

To sum up: YES! Use them! They are great and a good company to work with as well.

[kwaraska]

PCR Clean DX

We used them very successfully in my last lab. They even outperformed the originals.

[BioDynami]

SPRI Beads for DNA, RNA, microRNA & Oligo Purification

SPRI Beads (DNA & RNA Purification)
Optimized to purify DNA fragments of 100 bp and larger, and RNA fragments of 200 bases and larger.

Features:
 Purification of DNA and RNA

• DNA fragments > 100 bp
• RNA fragments > 200 nt

 Applications:

• NGS Library preparation
• PCR fragment purification
• DNA and RNA fragment purification
• Other applications requiring purified DNA and RNA

SPRI Beads (microRNA & Oligo Purification)
Overcomes the hurdle of the short DNA/RNA recovery problem. DNA/RNA fragments shorter than 100 base pairs can be effectively recovered.

Features:
 Purification of short DNA and RNA

• microRNA
• dsDNA fragments >20 bp
• ssDNA fragments >20 nt
• RNA fragments >20 nt
• DNA/RNA hybrid fragments >20 nt
• Oligo and chimeric oligo >20 nt

Learn More

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[BioDynami]

SPRI Beads (DNA & RNA Purification)
Optimized to purify DNA fragments of 100 bp and larger, and RNA fragments of 200 bases and larger.

Features:
 Purification of DNA and RNA

• DNA fragments > 100 bp
• RNA fragments > 200 nt

 Applications:

• NGS Library preparation
• PCR fragment purification
• DNA and RNA fragment purification
• Other applications requiring purified DNA and RNA

SPRI Beads (microRNA & Oligo Purification)
Overcomes the hurdle of the short DNA/RNA recovery problem. DNA/RNA fragments shorter than 100 base pairs can be effectively recovered.

Features:
 Purification of short DNA and RNA

• microRNA
• dsDNA fragments >20 bp
• ssDNA fragments >20 nt
• RNA fragments >20 nt
• DNA/RNA hybrid fragments >20 nt
• Oligo and chimeric oligo >20 nt

Learn More

-

[huguesparri]

CleanNGS are fine too.
They work both on DNA and RNA and are rather cheap.