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  • snapper
    Junior Member
    • Jun 2009
    • 5
    #1

    Bait tiling

    Hi,
    Does anyone have any experience of using the bait tiling function in the Agilent SureSelect target enrichment custom array design? What advantage does having a 2x or 3x bait tiling panel add?
    Thanks.
    Tags: None
  • upenn_ngs
    Member
    • Sep 2009
    • 70
    #2
    If the experiment will be conducted with single-end reads (i.e. 1x76bp), it is advantageous to have 2-3x tiling as the capture will be manufactured to include more probes per targeted loci. When using paired-end sequencing (2x76bp), the need to enrich more fragments is less because more data is collected per fragment.

    Comment

    • hbn
      Member
      • Apr 2011
      • 16
      #3
      I have a related question: I would like to do target enrichment using the SureSelect system for a non-model species.
      My question is; what software to use for this? Agilent advises me to use eArrayXD (local software) but I can not find this software anywhere...
      Does someone have experience with this? I would appreciate any advice...

      Comment

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