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  • fuellen
    Member
    • Jan 2018
    • 10

    RNA Sequencing "at home", when will this be easy?

    Hello,
    When will RNA-sequencing (gene expression) easily be possible at home, or at
    the doctor's office / point of care?
    Right now, it seems that sample preparation is still too demanding, while the sequencing itself is no longer the big problem.
    But maybe I'm wrong?
    Are there new developments coming up?
    "Easily possible" would mean that the doctor/nurse can perform all the necessary steps him/herself in a few minutes.
    Whether the sample is then also sequenced at the point of care, doesn't really matter; just freezing the sample (e.g. blood) doesn't look like a solution though, because the equipment for freezing to a sufficient temperature is usually not available, as far as I could find out.
    Thanks for any hints!

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    Yesterday, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

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