Overloading QiaAmp minelute virus spin columns

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Dorota

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Join Date: May 2017

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#1

Overloading QiaAmp minelute virus spin columns

03-02-2018, 05:56 AM

Hi,
did anyone try to include more volume than recommended 200uL on QiaAmp minelute virus spin kit columns ? As for exapmle just loading the lysed sample in two rounds on the column to bind? I want to load 400uL, human cells.
Any tips/suggestions? Did it have very negative impact on your yield?

(I know there are different kits, also QiaAmp vacuum, but I would really like to use the one mentioned above)
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Dorota

Junior Member

Join Date: May 2017

Posts: 9
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#2

05-09-2018, 03:53 AM

If anyone wonders, it kind of works :]
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