TruSeq adapter sequences

[kirankm]

Hi all,
My aim is to do targeted sequencing.
I wish to use the TruSeq DNA library prep kit for multiplexing and then use the Roche-Nilmblegen sequence capture method.

As Illumina has changed the protocol by adding the indexes to the adapters, I guess the oligos used in the Roche protocol (Hybridisation enhancement oligos etc) need to be modified.

The problem I do not have access to the Illumina TruSeq sequences namely; adapter-index oligos and the primer sequences.

Can some one share these sequences?

Thanks
KiranKM

[GW_OK]

I don't believe they are publically available. Our FAS says Illumina won't be providing them until sometime early next year, despite my protestations.

[aleferna]

still haven't ... frustrated grunt, anybody has the adapters for the Nextera V2?

[peromhc]

still haven't ... frustrated grunt, anybody has the adapters for the Nextera V2?

If you contact Illumina tech support, they will send them to you.

[aleferna]

I did, but they sent me a "letter to customer" pdf and I can't quite understand it, they have a RNA primers, DNA primers, some for oligo some for genomic dna, its a bit cryptic and if I get the wrong sequence it will be terrible expensive mistake.