Hi everybody
I am willing to sequence with MiSeq using a Nextera XT library starting with very challenging samples of not cultivable bacteria.
I am now checking the quality of my DNA after extraction (I can get a maximum of 16 ul of extract only).
I managed to quantify it with a high sensitivity kit (QBit measurement) and I currently know I have 1 ng DNA.
Does anybody know any efficient way to visualise 1 ng of DNA on gel?
I tried the classical protocol with 1% agarose and ethidium bromide and of course I couldn't see anything...
Any insight on how to check purity and fragmentation on such small DNA quantities?
Thanks a lot
Chiara
I am willing to sequence with MiSeq using a Nextera XT library starting with very challenging samples of not cultivable bacteria.
I am now checking the quality of my DNA after extraction (I can get a maximum of 16 ul of extract only).
I managed to quantify it with a high sensitivity kit (QBit measurement) and I currently know I have 1 ng DNA.
Does anybody know any efficient way to visualise 1 ng of DNA on gel?
I tried the classical protocol with 1% agarose and ethidium bromide and of course I couldn't see anything...
Any insight on how to check purity and fragmentation on such small DNA quantities?
Thanks a lot
Chiara
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