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  • SuzuBell
    Member
    • Nov 2013
    • 22
    #1

    Metagenome analysis from low biomass samples

    I have access to a sample that we hope to run a microbial ecological study on. The problem is that each sample only has about one picogram of DNA.

    What are the best molecular biology and/or bioinformatics techniques to look into to maximize the chance I can develop RNA-sequencing analysis techniques from such low biomass samples?

    Thank you for sharing any advice!
    Last edited by SuzuBell; 06-18-2018, 01:46 PM.
    Tags: None
  • nucacidhunter
    Jafar Jabbari
    • Jan 2013
    • 1250
    #2
    I do not think that there is any hyper sensitive method yet to preprare RNA-Seq library from 1 pg RNA.

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