Metagenome analysis from low biomass samples

[SuzuBell]

I have access to a sample that we hope to run a microbial ecological study on. The problem is that each sample only has about one picogram of DNA.

What are the best molecular biology and/or bioinformatics techniques to look into to maximize the chance I can develop RNA-sequencing analysis techniques from such low biomass samples?

Thank you for sharing any advice!

[nucacidhunter]

I do not think that there is any hyper sensitive method yet to preprare RNA-Seq library from 1 pg RNA.