I am hoping to collect samples in environments that have very little nutrients (such as a clean room in a hospital). The amount of DNA that is collected from these environments tends to be pretty small (about 1 picogram) and I hope to have enough RNA from it to perform a RNA-sequencing analysis on it related to microbial ecology.
What may be the best approach to the actual sample collection that could maximize the amount of DNA that I obtain? Sample from floor? Sample from air? And many other factors I may not be thinking of. I have tried to find successful examples of this type of sample collection but have not found much information yet (I might be using the wrong search terms).
I do not have much experience collecting DNA for this type of analysis, and would be grateful for any ideas or references on how to approach it! Thank you!
What may be the best approach to the actual sample collection that could maximize the amount of DNA that I obtain? Sample from floor? Sample from air? And many other factors I may not be thinking of. I have tried to find successful examples of this type of sample collection but have not found much information yet (I might be using the wrong search terms).
I do not have much experience collecting DNA for this type of analysis, and would be grateful for any ideas or references on how to approach it! Thank you!