Agencourt Ampure purification kit - exp date

[ntremblay]

Hi,

does anybody has experienced with an expired ampure kit?

the product datasheet says that the yield is consistent throughout the 12 months shelf life, but I wonder how much it impacts the performance afterward,

thanks for any information,

Nick

[ParkerKB]

I know this is an old post but just came across it...I haven't tried this yet but a number of other posts suggest titrating your ampure beads using a DNA ladder/marker. See the Roche technical bulletin 2011-002 (attached). That would give you an idea of how functional the beads are after expiring.

[mboth]

I have just run a test on our AMPure XP beads, between new beads with ones that were 4 months over their expiry date. I compared different titrations on a ladder, and saw no difference whatsoever!

[TonyBrooks]

It's hit and miss for us.
Sometimes they work well, sometimes we've run samples with out of date beads and lost everything. I guess some of it may depend on how many times they've been used (i.e. brought up to room temp and cooled again).