Our lab has built a drop-seq setup. Going over the protocol, I see that the cells are lysed in a solution of sarkosyl, Ficoll-400, tris, EDTA, and DTT. I'm not intimately familiar with the biochemstry of this lysis buffer. I'm wondering if it is effective at inactivating endogenous RNAses? I haven't found anyone online that has discussed degradation of RNA at this stage. But the pumping can take quite a while, depending on the scale of the experiment (at least 15 minutes per sample). I am just curious if this is something I should or shouldn't be concerned about.
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...09-01-2026, 05:41 AM