Our lab has built a drop-seq setup. Going over the protocol, I see that the cells are lysed in a solution of sarkosyl, Ficoll-400, tris, EDTA, and DTT. I'm not intimately familiar with the biochemstry of this lysis buffer. I'm wondering if it is effective at inactivating endogenous RNAses? I haven't found anyone online that has discussed degradation of RNA at this stage. But the pumping can take quite a while, depending on the scale of the experiment (at least 15 minutes per sample). I am just curious if this is something I should or shouldn't be concerned about.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM