Illumina is going to re-introduce ribozero equivalent kits in 2020.
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Originally posted by cement_head View PostHas anyone tried this kit from ZYMO? Usually ZYMO stuff is pretty good...
https://www.zymoresearch.com/product...ersal-cdna-kit
We have options for people who are interested in doing probe-free depletion. Contact [email protected] and tell them you'd like to try the RiboFree Total RNA Library Kit. We were offering trial sizes and free samples a few months ago.
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Illumina did release a new version of RiboZero+ yesterday: https://www.illumina.com/products/by...depletion.html
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Originally posted by jjlin View PostDISCLAIMER: I work for Zymo
We have options for people who are interested in doing probe-free depletion. Contact [email protected] and tell them you'd like to try the RiboFree Total RNA Library Kit. We were offering trial sizes and free samples a few months ago.
This would make it species agnostic. Specified to work down to 100ng or somewhat below that with extended hybridization times.
Most of the charm of the old Truseq Ribozero-depletion library kits was that although they specified a limited range of species, eg "human/mouse/rats" the probes were very long (not oligos) and so actually seemed to work on any animal. For example they worked on moth RNA in our hands.
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Phillip
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