Illumina is going to re-introduce ribozero equivalent kits in 2020.
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For those seeking a bacterial rRNA depletion kit, NEB has just released a kit that works very well in our hands.
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DISCLAIMER: I work for ZymoOriginally posted by cement_head View Post
We have options for people who are interested in doing probe-free depletion. Contact [email protected] and tell them you'd like to try the RiboFree Total RNA Library Kit. We were offering trial sizes and free samples a few months ago.Comment
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According to rumors, RiboZero will make a comeback. Not exactly the same products though; it will use a new chemistry.Comment
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Illumina did release a new version of RiboZero+ yesterday: https://www.illumina.com/products/by...depletion.htmlComment
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Interesting. Appears to use a strand-denaturation/hybridization-based normalization to remove/reduce concentrations of highly represented cDNAs. That is, enzymatic digestion of DNA/RNA duplexes that form due to C0t kinetics.Originally posted by jjlin View Post
This would make it species agnostic. Specified to work down to 100ng or somewhat below that with extended hybridization times.
Most of the charm of the old Truseq Ribozero-depletion library kits was that although they specified a limited range of species, eg "human/mouse/rats" the probes were very long (not oligos) and so actually seemed to work on any animal. For example they worked on moth RNA in our hands.
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PhillipComment
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Thanks. No manual available yet. List price is $82 per reaction.
Originally posted by GenoMax View PostComment
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
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