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  • sdirienzi
    Junior Member
    • Jan 2019
    • 1

    #1

    Direct Bacterial Colony Sequencing with the Nextera DNA Flex

    Has anyone tried out Illumina’s protocol for Direct Bacterial Colony Sequencing with the Nextera DNA Flex Library Preparation Kit? I'm interested in using the protocol for Lactobacillus, Streptococcus, Prevotella, and Veillonella species.

    Thanks!
    Sara

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  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
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    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
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