Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • bcf
    Junior Member
    • Mar 2009
    • 2

    #1

    Edit distance sequence tags available (link within)

    Hi everyone,

    I've generated several sets of edit (levenshtein) distance/metric sequence tags from 4nt to 10nt and edit distance 3 to edit distance 9. Within each sheet of the spreadsheet (or length/edit distance combination in csv format), all tags are at least the given edit distance from one other.

    While not entirely exhaustive (for several reasons), these sequence tags approach the maximum number possible (after filtering) within each category of length.

    I'm in the process of prepping a manuscript describing the code for tag generation (as part of a larger package on which i'm working - largely in a private branch of the repo.), but it seemed these tags might be useful to others in the meantime.

    the tags are available from:



    there's a blog post with a bit of description here:



    and the code to generate the tags is here:



    I generated the tags in the files provided using the following (to generate 10nt tags with a minimum edit distance of 3, for example):

    Code:
    python make_levenshtein_tags.py --tag-length=10 --edit-distance=3 \
    --no-polybase --gc --comp --use-c --multiprocessing \
    --min-and-greater | tee 10_nt.txt
    Run time for this example was 80 hours or so on 6 cores of 8 (Mac Pro, 8 GB RAM) or approx. 480 hours wall-clock time. Run times for smaller tag sets (e.g. 8 nt) are about a minute.

    if you use these tags, for the time being, please "cite" the repository link (https://github.com/baddna/edittag/). Once we get a manuscript out, I'll provide proper citation information in the README.

    WORDS OF CAUTION: i'm undertaking a re-organization of the code in this repository, committing lots of unit tests, refactoring, documenting, etc. - so expect the code to change as i update and refactor.

    please let me know if you have any questions.

    best,
    b
    Last edited by bcf; 01-18-2011, 10:58 PM. Reason: Updating URLs for repository move
  • bcf
    Junior Member
    • Mar 2009
    • 2

    #2
    edittag packaged up and on pypi

    Hi everyone,

    two quick things:


    -b
    Last edited by bcf; 02-14-2011, 08:37 AM. Reason: preprint is now accessible

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
      by SEQadmin2



      CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

      Despite this, “CRISPR helped turn genome editing from a specialized technique into
      ...
      07-31-2026, 11:01 AM
    • SEQadmin2
      Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
      by SEQadmin2


      Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

      The systematic characterization of the human proteome has
      ...
      07-20-2026, 11:48 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 08-11-2026, 10:35 AM
    0 responses
    10 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 08-06-2026, 07:41 AM
    0 responses
    29 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 08-03-2026, 10:13 AM
    0 responses
    47 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-31-2026, 02:55 AM
    0 responses
    48 views
    0 reactions
    Last Post SEQadmin2  
    Working...