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Has anyone used AMPureXP beads older than 18 months, (assuming that they were stored properly)?
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Hi there,
Off the top of my head I think I am using a bottle, stored at +4, that is currently sitting older than 18 months and they have performed perfectly in all my recent preps so I'd say you're good to go! -
I used AmpPure beads ~ + 4 month. They have worked. I have checked mine first with a not important sample. You can test yours with a DNA ladder. I think it is in general a good idea.Comment
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I have sometimes used Ampure beads that were 1-3 years past expiration date. They sort of work, but they seem to become less magnetic and and are therefore more difficult to work with.Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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