Hey Guys,
Does anyone have first hand experience of amplifying ChIP'd DNA before preparing an Illumina library? For reasons I will not go into hear I need to amplify my material. I've seen suggestions of a protocol involving amplification followed by concatenation of the amplified fragments and subsequent fragmentation of the concatenated DNA. But have had difficulty finding somebody with first hand experience whose brain I can pick If anyone has any experience I'd really appreciate some help
Cheers
Optimus
Does anyone have first hand experience of amplifying ChIP'd DNA before preparing an Illumina library? For reasons I will not go into hear I need to amplify my material. I've seen suggestions of a protocol involving amplification followed by concatenation of the amplified fragments and subsequent fragmentation of the concatenated DNA. But have had difficulty finding somebody with first hand experience whose brain I can pick If anyone has any experience I'd really appreciate some help
Cheers
Optimus
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