I've been looking into the Illumina TST170 sample prep kit. Reading the protocol, you either make a sheared DNA library or a cDNA library from RNA, then both go into the hybridisation capture based. But the data sheet says that fusions and splice variants can only be detected from RNA for a subset of the 170 genes. Why is this, if they are all going into the same capture?
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM