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I recently obtained strange results from a ChIP-seq experiment. Rather than having relatively thorough coverage of the genome, my reads were separated by large gaps and many were identical to each other and stacked right on top of each other. (I should note that I did also see enrichment at predicted areas.) We have not observed this pattern before. Then only thing that we see abnormal in the Illumina sequencer output info is that there was a large bias for G as the first nucleotide in the sequences. Could be related to the large stacks of identical reads? Any thoughts on why this may be occuring? Do you think it could be an inefficient ligation or amplification issue? TIA!
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...Yesterday, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM