I recently submitted the GecKO library (~120K guides) for a MiSeq Nano run to check diversity (and mostly to check my custom seq primer etc. was working). It performed 1.3M reads, so about 10x the library size. I realize this is not nearly enough, since it's recommended to do 100x the library size. Even with 1/10th of the recommended depth, I had about 2.5% unread guides (rec is <0.5%). Do you think I can go ahead with this library? I really don't want to spend more money to do a 12M+ run. Thoughts?
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...