Has anybody got any experience in pooling 1000 barcodes?
I am planning a nested multiplex PCR, testing several loci in 1000 clonal colonies for indels. Second PCR should introduce barcodes. IDT have a list, but before I go picking, is there anything I need to consider for compatibility? Has anyone optimised this type of thing?
I am planning a nested multiplex PCR, testing several loci in 1000 clonal colonies for indels. Second PCR should introduce barcodes. IDT have a list, but before I go picking, is there anything I need to consider for compatibility? Has anyone optimised this type of thing?