Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • #1

    Cheaper library prep service provider?

    Hello,
    there are multiple published manners of getting much cheaper library preparations if you do them in-house. That's great if you have a qualified tech who has trained to do this on staff or time/skills to do it yourself. However for some of us that's not the case!

    Are there any service providers that will do RNAseq preps at e.g. 1/10th volume or using one of the other published tricks? Library prep costs are still a killer for many projects....

    Thanks!
    Yannick
    Tags: dna, illumina, library preparation, rnaseq, service provider
  • #2
    if you want whole genome resequencing, I can help!
    All-in-one sequencing: an improved library preparation method for cost-effective and high-throughput next-generation sequencing - Plant Methods
    https://plantmethods.biomedcentral.com/articles/10.1186/s13007-020-00615-3
    Background Next generation sequencing (NGS) has been widely used in biological research, due to its rapid decrease in cost and increasing ability to generate data. However, while the sequence generation step has seen many improvements over time, the library preparation step has not, resulting in low-efficiency library preparation methods, especially for the most time-consuming and labor-intensive steps: size-selection and quantification. Consequently, there can be bottlenecks in projects with large sample cohorts. Results We have described the all-in-one sequencing (AIO-seq) method, where instead of performing size-selection and quantification for samples individually, one sample one tube, up to 116 samples are pooled and analyzed in a single tube, ‘All-In-One’. The AIO-seq method pools libraries based on the samples’ expected data yields and the calculated concentrations of the size selected regions (target region), which can easily be obtained with the Agilent 2100 Bioanalyzer and Qubit Fluorometer. AIO-seq was applied to whole genome sequencing and RNA-seq libraries successfully, and it is envisaged that it could be applied to any type of NGS library, such as chromatin immunoprecipitation coupled with massively parallel sequencing, assays for transposase-accessible chromatin with high-throughput sequencing, and high-throughput chromosome conformation capture. We also demonstrated that for genetic population samples with low coverage sequences, like recombinant inbred lines (RIL), AIO-seq could be further simplified, by mixing the libraries immediately after PCR, without calculating the target region concentrations. Conclusions The AIO-seq method is thus labor saving and cost effective, and suitable for projects with large sample cohorts, like those used in plant breeding or population genetics research.

    Comment

    Previous template Next

    Latest Articles

    Collapse
    • seqadmin
      Recent Advances in Sequencing Analysis Tools
      by seqadmin


      The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
      Today, 07:48 AM
    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      04-22-2024, 07:01 AM
    View All

    ad_right_rmr

    Collapse

    News

    Collapse
    Topics Statistics Last Post
    Enhanced Neoantigen Detection: Introducing NeoHunter by seqadmin
    Started by seqadmin, Today, 07:17 AM
    0 responses
    11 views
    0 likes
    		 Last Post seqadmin
    by seqadmin
    Today, 07:17 AM  
    A Close Examination at Probiotic-Related Bacteremia by seqadmin
    Started by seqadmin, 05-02-2024, 08:06 AM
    0 responses
    19 views
    0 likes
    		 Last Post seqadmin
    by seqadmin
    05-02-2024, 08:06 AM  
    Expanded Genetic Insights into Blood Pressure Regulation by seqadmin
    Started by seqadmin, 04-30-2024, 12:17 PM
    0 responses
    20 views
    0 likes
    		 Last Post seqadmin
    by seqadmin
    04-30-2024, 12:17 PM  
    The Role of Enhancers in Defining Cell Fate by seqadmin
    Started by seqadmin, 04-29-2024, 10:49 AM
    0 responses
    28 views
    0 likes
    		 Last Post seqadmin
    by seqadmin
    04-29-2024, 10:49 AM  
    View All
    Working...
    X