"Soft" Nuclear Lysis + PCR (ATAC-SEQ)

fderop

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"Soft" Nuclear Lysis + PCR (ATAC-SEQ)

05-25-2020, 10:07 AM

Hi all,

I am interested in performing ATAC-seq in a confined space. I tagment the nuclei first, then isolate them, and then I want to perform a PCR on these nuclei. However, how would I lyse the nuclei/denature Tn5 without inhibiting the subsequent PCR? Washing inbetween is not an option for us sadly. Do any of you have any tips for this "soft" lysis that does not hamper PCR?
Tags: atac-seq, lysis, nuclei

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