I prepped my final indexed libraries two different ways (essentially SmartSeq2 vs Smartseq 3) and got vastly different final preps after index PCR. Which one would be more suitable for sequencing? Obviously a first-timer here!
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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