Hi,
Before sending in a sample for 6kb Mate pair library preparation what should be the size range of the DNA?
I have extracted Eukaryotic DNA and run it beside a high range ladder (the highest marker is at 48KB). However,it shows a 'light' smear upto the 20kb marker.
Gel picture attached
Is this DNA too fragmented for Mate pair library prep? I have tried Qiagen kit and phenol chloroform extractions. What is the best protocol to avoid fragmentation?
Thanks
Before sending in a sample for 6kb Mate pair library preparation what should be the size range of the DNA?
I have extracted Eukaryotic DNA and run it beside a high range ladder (the highest marker is at 48KB). However,it shows a 'light' smear upto the 20kb marker.
Gel picture attached
Is this DNA too fragmented for Mate pair library prep? I have tried Qiagen kit and phenol chloroform extractions. What is the best protocol to avoid fragmentation?
Thanks
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