Hi All,
I know different libraries will cluster more or less efficiently due to the size of the library molecules, GC content etc., etc.
I'm interested in how much variation (in cluster density) is observed between libraries when clustered at the same concentration as measured by qPCR.
If you use qPCR, how are you 'calibrating' your measurements for concentration to cluster density? Do you maintain a variety of GC content libraries, titrated on flowcells, or just use a single library (such as PhiX)?
How reliable do you find the qPCR measurements in terms of achieving the targeted cluster density?
Cheers,
Scott.
I know different libraries will cluster more or less efficiently due to the size of the library molecules, GC content etc., etc.
I'm interested in how much variation (in cluster density) is observed between libraries when clustered at the same concentration as measured by qPCR.
If you use qPCR, how are you 'calibrating' your measurements for concentration to cluster density? Do you maintain a variety of GC content libraries, titrated on flowcells, or just use a single library (such as PhiX)?
How reliable do you find the qPCR measurements in terms of achieving the targeted cluster density?
Cheers,
Scott.