We have successfully reduced the volumes of the reagents of the Illumina DNA prep kit to 1/4. We want to further reduce the cost. Is there any way to dilute the reagent (especially BLT beads since the reagent has the lowest volume) instead of further reducing the volumes?
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It can be done and dilution factor depends on the quantity of input DNA (if using purified DNA) for up to 3 ng input BLT can be safely diluted 40x. Add water to make up for reduced BLT volume. There is also a preprint: https://www.biorxiv.org/content/bior...79215.full.pdf -
Hi, would it be possible to know how? is it accessible.Originally posted by szy0931 View Post
thanks
M.Comment
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It is described in detail in the Hackflex paper.
The latest version of the manuscript is here: https://www.biorxiv.org/content/10.1101/779215v2Comment
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