We have successfully reduced the volumes of the reagents of the Illumina DNA prep kit to 1/4. We want to further reduce the cost. Is there any way to dilute the reagent (especially BLT beads since the reagent has the lowest volume) instead of further reducing the volumes?
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It can be done and dilution factor depends on the quantity of input DNA (if using purified DNA) for up to 3 ng input BLT can be safely diluted 40x. Add water to make up for reduced BLT volume. There is also a preprint: https://www.biorxiv.org/content/bior...79215.full.pdf
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Originally posted by szy0931 View PostWe have successfully reduced the volumes of the reagents of the Illumina DNA prep kit to 1/4. We want to further reduce the cost. Is there any way to dilute the reagent (especially BLT beads since the reagent has the lowest volume) instead of further reducing the volumes?
thanks
M.
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It is described in detail in the Hackflex paper.
The latest version of the manuscript is here: https://www.biorxiv.org/content/10.1101/779215v2
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