Can anyone recommend alternatives to the SequalPrep Normalization Kit (cat# A1051001) for normalization of amplicon sample concentration for MiSeq? The kit is currently backordered until late August (2021).
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There are several brands of normalization beads as alternative. I like product from Aline Biosciences https://alinebiosciences.com/product/dna-normalizer-v3/. -
This seems to be 1:1 substitute for SequalPrep?
We have no experience with it, though.Comment
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Has anyone tried this one: ChargeSwitch™ PCR Clean-Up Kit (thermofisher.com)​? Works on the same principle as the SequalPrep but on magnetic beadsComment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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