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  • Master-Mix
    Junior Member
    • Oct 2021
    • 1
    #1

    TN5 with costume oligos?

    I would like to create my custom oligos fused to TN5 to use to create NGS libraries. Douse anyone know a company who would provide this service? I could see there are protocols to do this on your own. Maybe someone is out there who could answer some question regarding this oligos.
    What is the maximum sequence length which could be introduced efficiently by TN5?
    Illumina offers the bead bound TN5 to control the library length (nextera flex?). Is it possible to get different oligo sequences for the bead bound TN5 as well
    Thanks in advance for your help!!!
    Tags: None
  • huguesparri
    Member
    • May 2008
    • 97
    #2
    Hello,

    You can purchase "naked" tn5 from Diagenode and fill it with the oligos you need.
    Unfortunately, I can't answer your other questions :s
    Regards,

    H.

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