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Hi @egotistdown, I am wondering whether you figured out the reason. I encountered the same issue. Thanks.
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10x 5' GEX - overrepresentation of RT Primer in results
Hi all -
First time post so apologies if this is the wrong spot..!
I'm having issues with my first 10x 5' gene expression run and am looking for help troubleshooting what went wrong.
The sequencing resulted in a low percentage of reads mapping to the transcriptome (~30%). Further investigation revealed an over-expression of RT Primer in Read 2. Read 1 appeared fine.
We loaded 8000 cells and detected 6000-9000 cells (in 4 samples). Knee-plots looked good, with not much background or degraded RNA. Bioanalyzer traces taken after cDNA amplification and after library completion both looked ok.
Samples were PBMCs treated for 18 hours with exosomes.
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by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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04-04-2024, 04:25 PM -
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by seqadmin
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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03-22-2024, 06:39 AM -
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