Fragment Analyser HS NGS Frag 1-6000 profile

[Sbiljh1]

I'm looking for some help analysing the profile of a 10x genomics 3' scRNA library prep, run on a 1-6000bp HS fragment analyser (attached pic). The second of my libraries doesn't have a 'smooth' profile and I was hoping for some insight as to why this might be! Will it affect sequencing?

For reference, this data was obtained from differentiating hiPSCs at different time-points.

Thanks!

https://ibb.co/jM0yqQ7

[Epimed]

Hello,

I am having the same profile of the second fragment analyzer peek, where it is split into two.

Did you manage to find any answer or good interpretation?

thank you!