Do proteins interfere with NGS or lib prep??

hmviit

Junior Member

Join Date: May 2011

Posts: 2
- Share
- Tweet
#1

Do proteins interfere with NGS or lib prep??

05-02-2011, 10:29 PM

Hi all,

I found this forum a week a ago and it has turned out to be useful. The only thing I'm still wondering about is said in the title. I will be doing DNA sequencing on 454 after seq capturing. I suspect that the gDNA that I have after extraction has RNA as a contamination (liver tissue so I expect RNA) and I want to treat the gDNA with RNAse A. But after that I'm not sure how important it is to get rid of the RNAse in the gDNA and by serching around I haven't found a good answer. I'm using salt-extraction and dissolving my gDNA in to H2O. So after treating with RNAse, should I re-extract or just precipitate to get rid of the RNAse-proteins or just let it be?

Thanks,
Heidi
Tags: None
gogreen

Member

Join Date: Apr 2009

Posts: 18
- Share
- Tweet
#2

05-08-2011, 06:05 AM

Hi Heidi, I would recommend a cleanup of gDNA (columns, beads or phenol ChCl3) before you do library prep. If I got it right, you want to use the DNA for sequence capture before actual sequencing. Having clean DNA in first place would already help a lot in the downstream.
Comment

Previous template Next

Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications

by SEQadmin2

Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

The systematic characterization of the human proteome has...
- Channel: Articles
07-20-2026, 11:48 AM
Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems

by SEQadmin2

Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
...
- Channel: Articles
07-09-2026, 11:10 AM
Cancer Drug Resistance: The Lingering Barrier to Rising Survival

by SEQadmin2

Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
- Channel: Articles
07-08-2026, 05:17 AM

Topics	Statistics	Last Post
Chemotherapy Leaves Detectable DNA Signatures in Childhood Tumors by SEQadmin2 Started by SEQadmin2, Today, 11:41 AM	0 responses 9 views 0 reactions	Last Post by SEQadmin2 Today, 11:41 AM
Single-Cell Atlases Skew Toward European Ancestry, Analysis Finds by SEQadmin2 Started by SEQadmin2, 07-20-2026, 11:10 AM	0 responses 21 views 0 reactions	Last Post by SEQadmin2 07-20-2026, 11:10 AM
UC San Diego Bioengineers Map Gene Function in Human Stem Cells by SEQadmin2 Started by SEQadmin2, 07-13-2026, 10:26 AM	0 responses 34 views 0 reactions	Last Post by SEQadmin2 07-13-2026, 10:26 AM
New Analysis Splits Leukemia Into 16 Epigenomic Subgroups by SEQadmin2 Started by SEQadmin2, 07-09-2026, 10:04 AM	0 responses 44 views 0 reactions	Last Post by SEQadmin2 07-09-2026, 10:04 AM

Unconfigured Ad

Do proteins interfere with NGS or lib prep??

Comment

Latest Articles

ad_right_rmr

News