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  • Jon_Keats
    Senior Member
    • Mar 2010
    • 279

    #16
    Has anyone taken a TRuseq library and amplified with PPC and custom primers and checked the library size by bioanalyzer? IF the PPC are really longer the libraries should be slightly bigger I'd think?

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    • pmiguel
      Senior Member
      • Aug 2008
      • 2328

      #17
      Originally posted by Jon_Keats View Post
      Has anyone taken a TRuseq library and amplified with PPC and custom primers and checked the library size by bioanalyzer? IF the PPC are really longer the libraries should be slightly bigger I'd think?
      I was taking that position earlier. However my championing of this possibility pretty much ended because of arguments and evidence supplied in this thread by csquared. (Although I would love to see mass spec results -- did you ever try that Ethan?)

      --
      Phillip

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