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Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Started by aleichter, 08-07-2017, 07:37 PM
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3 responses
1,761 views
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by fkrueger
08-10-2017, 01:51 AM
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Started by seqtechno1, 08-09-2017, 04:59 AM
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0 responses
1,569 views
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by seqtechno1
08-09-2017, 04:59 AM
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Started by candida utilis, 08-07-2017, 07:44 AM
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2 responses
2,722 views
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by jteeee2
08-08-2017, 08:37 AM
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Started by barn88, 07-28-2017, 07:26 AM
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2 responses
3,987 views
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by barn88
08-05-2017, 09:57 AM
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Started by Arthur45, 07-04-2017, 06:05 AM
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1 response
2,488 views
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by Arthur45
08-04-2017, 08:42 AM
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Started by SS00, 03-24-2014, 05:16 AM
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6 responses
10,878 views
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by patkrat
08-04-2017, 03:11 AM
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Started by jpleonard2000, 07-29-2015, 10:38 AM
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7 responses
4,136 views
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by GMDickson
08-02-2017, 04:23 AM
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Started by Kaizen, 07-31-2017, 12:00 AM
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0 responses
3,414 views
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by Kaizen
07-31-2017, 12:00 AM
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Started by mia2025, 07-26-2017, 06:20 AM
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7 responses
3,236 views
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by mia2025
07-28-2017, 06:11 AM
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Started by PrimerBuffalo, 04-11-2017, 09:23 AM
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6 responses
3,109 views
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Last Post
by Simone78
07-21-2017, 08:14 AM
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by SEQadmin2
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...-
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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07-01-2026, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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Started by SEQadmin2, Yesterday, 10:08 AM
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by SEQadmin2
Yesterday, 10:08 AM
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Started by SEQadmin2, 07-07-2026, 11:05 AM
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07-07-2026, 11:05 AM
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Started by SEQadmin2, 07-02-2026, 11:08 AM
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07-02-2026, 11:08 AM
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