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  • malonzm1
    Member
    • Mar 2022
    • 10

    #1

    PHLAT genotyping with scRNA-seq (10x)

    Hi,

    Can PHLAT (HLA genotyping) be used with single cell RNA-seq (e.g. 10x platform)?

    Thanks and good day.
    ​
  • GenomicSeq
    Member
    • Oct 2022
    • 39

    #2
    malonzm1 you should be able to do this. I thought I saw a pre-print on something like this. I can't remember if it's this one (https://www.biorxiv.org/content/10.1...06.09.495569v1) or not. Maybe I can dig it up if this doesn't look right to you.

    Comment

    • malonzm1
      Member
      • Mar 2022
      • 10

      #3
      Hi @GenomicSeq,

      Thanks! Would you know if it requires first demultiplexing the 10x fastq files?

      Comment

      • malonzm1
        Member
        • Mar 2022
        • 10

        #4
        What about smart-seq2 and fluidigm C1?

        Comment

        • GenomicSeq
          Member
          • Oct 2022
          • 39

          #5
          malonzm1 sorry for the late response. I just realized you replied. But yeah you would have to demultiplex them. Although I'm not super familiar with the Fluidigm C1. What specifically is your question with that?

          Comment

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