Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 1
  • Time
  • Show
Clear All
new posts
Previous template Next
  • feng
    Member
    • Oct 2010
    • 50
    #1

    statistical analysis

    How to do statistical analysis on differentially expressed genes from RNA-seq?

    Results as


    genes Sample1 Sample2
    xxxx 12 23
    xxxx 155 30

    Numbers stand for times of gene sequence.
    Tags: None
    • Bukowski
      Senior Member
      • Jan 2010
      • 388
      #2
      Originally posted by feng View Post
      How to do statistical analysis on differentially expressed genes from RNA-seq?

      Results as


      genes Sample1 Sample2
      xxxx 12 23
      xxxx 155 30

      Numbers stand for times of gene sequence.
      Not much chance of statistical analysis with only two samples.

      I think DESeq http://www-huber.embl.de/users/anders/DESeq/ has instructions for unreplicated conditions though. If you search the forums there are plenty of discussions on SeqAnswers about this topic.

        Comment

        • ickou
          Junior Member
          • Aug 2010
          • 8
          #3
          You can use "NOISeq-sim: no replicates"
          tutorial [NOISeq]
          http://bioinfo.cipf.es/noiseq/doku.php?id=tutorial
          tutorial

            Comment

            Previous template Next

            Latest Articles

            Collapse
            • SEQadmin2
              Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
              by SEQadmin2



              Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

              Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating              ...
              10-14-2026, 09:55 AM
            • SEQadmin2
              New Genomics Technologies Take Aim at Long-Standing Limits
              by SEQadmin2


              Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

              We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing              ...
              09-28-2026, 10:25 AM
            View All

            ad_right_rmr

            Collapse

            News

            Collapse
            Topics Statistics Last Post
            Exeter-Led Study Maps What an Average Human Genome Looks Like by SEQadmin2
            Started by SEQadmin2, Yesterday, 11:13 AM
            0 responses
            16 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            Yesterday, 11:13 AM  
            Long-Read Sequencing Uncovers Cancer Mutations Conventional Testing Missed by SEQadmin2
            Started by SEQadmin2, Yesterday, 10:48 AM
            0 responses
            18 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            Yesterday, 10:48 AM  
            Mayo Clinic-Led Study Detects DNA Mutations Across Noncancerous Tissues by SEQadmin2
            Started by SEQadmin2, 10-15-2026, 10:52 AM
            0 responses
            35 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            10-15-2026, 10:52 AM  
            Study Finds Chromatin Motion Holds Steady Until Cells Face Stress by SEQadmin2
            Started by SEQadmin2, 10-14-2026, 05:39 AM
            0 responses
            42 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            10-14-2026, 05:39 AM  
            View All
            Working...