Hello All,
I am an undergraduate student, and am fortunate enough to get to work on a gut metagenomics project this summer. Before I get to work on our dataset, I would like to work on a couple of datasets that were uploaded to the sequence read archive for practise. I have downloaded the SRA files from a similar project, but I am confused how to separate each sample in the run according to its barcode/primer combination. I found the primers in the original paper, but I have no idea how to get the barcodes. I don't see them listed on the study's page:
Does anyone know if they are stored in the SRA file metadata, and if there is a way so separate the samples by barcode? I looked through the fastq-dump parameters for the SRA toolkit, but I don't see anything that will do that. Any help will be appreciated!
Thanks in advance.
I am an undergraduate student, and am fortunate enough to get to work on a gut metagenomics project this summer. Before I get to work on our dataset, I would like to work on a couple of datasets that were uploaded to the sequence read archive for practise. I have downloaded the SRA files from a similar project, but I am confused how to separate each sample in the run according to its barcode/primer combination. I found the primers in the original paper, but I have no idea how to get the barcodes. I don't see them listed on the study's page:
Does anyone know if they are stored in the SRA file metadata, and if there is a way so separate the samples by barcode? I looked through the fastq-dump parameters for the SRA toolkit, but I don't see anything that will do that. Any help will be appreciated!
Thanks in advance.