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  • Chuckytah
    Member
    • Mar 2011
    • 65
    #1

    From Fasta get GO-terms

    Hello friends,

    I used to do GO annotation using Blast2GO but it strangely gave me 0 GO-terms to a 507 sequences dataset... it was really strange... The blast went fine, but when I try the annotation it gives me no hits at all... I dunno if I made me clear.

    You know any other software to have GO-terms from fasta files?

    Thanks.
    Tags: blast, gene annotation, gene ontology, go terms
    • JackieBadger
      Senior Member
      • Mar 2009
      • 385
      #2
      did you change the type of blast search? GO annotations only work with BLASTx.... tBLASTx does not work

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        • Chuckytah
          Member
          • Mar 2011
          • 65
          #3
          Originally posted by JackieBadger View Post
          did you change the type of blast search? GO annotations only work with BLASTx.... tBLASTx does not work
          I really can't remember if I did any alteration in the parameters. lol but I already solve my problem in tab Tools there is an option that "Restore all original B2G properties", and now everything is okay.

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