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We have put out a "final" release of BEDOPS v2.1 here, which includes the psl2bed script.
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I have posted a new psl2bed conversion script to an unstable development branch of our BEDOPS suite, which you can download as follows:
$ svn checkout http://bedops.googlecode.com/svn/branches/v2p1p0 bedops_v2p1p0
$ cd bedops_v2p1p0
$ make
$ make install
$ cd bin
$ ./psl2bed --help
This script will convert headerless (default) and headered PSL files by moving columns around into BED output that follows UCSC BED format conventions, up to the first six columns. Remaining fields are mapped to columns 7 through 21.
Run psl2bed --help for a more complete description.
As an example, here is how to make a sorted BED file called sorted-foo.bed, converted from a headered PSL file called foo.psl:
$ psl2bed --headered < foo.psl > sorted-foo.bed
This relies on installing BEDOPS binaries, including psl2bed and sort-bed in a location accessible through your PATH environment variable.
Note that this is a development branch, so there are no guarantees that versions of BEDOPS apps made from this branch are stable. These and other improvements will end up in the final v2.1 release of BEDOPS, hopefully in a month or two.Last edited by AlexReynolds; 03-09-2013, 03:06 AM.
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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Channel: Articles
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by SEQadmin2
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Here are nine questions we think about, in roughly the order they matter, before...-
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