Kind of a beginner question here which is not specific to any particular organism. To train GlimmerHMM you need a file with the genes, and a file with the coordinates of the exons within those genes. The genes are downloadable from NCBI, but I am wondering how you get the exon coordinates. Are they also downloadable from NCBI? I feel like they should be, otherwise they wouldn't have the genes available if they didn't have some idea of the intron-exon boundaries within the genes. Or is the availability of the exon information dependent on the degree of annotation of the organism?
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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