Hi people!
We assembled a de novo assembly using deep PE reads. Now we want to run biological replicates to analyse DE. Naively, I thought we should run he very same PE sequencing on those replicates, but I was advised by the people using illumina that, as my transcriptome is assembled and seems to be of good quality, I could go for SE sequencing. It'd save me a lot of money for same results. What would you think about that? Thanks!
Kate
We assembled a de novo assembly using deep PE reads. Now we want to run biological replicates to analyse DE. Naively, I thought we should run he very same PE sequencing on those replicates, but I was advised by the people using illumina that, as my transcriptome is assembled and seems to be of good quality, I could go for SE sequencing. It'd save me a lot of money for same results. What would you think about that? Thanks!
Kate
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