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  • Aligner that reports all alignments?

    Hi, I'm looking for an aligner that reports multiple/all hits rather than just a single one. I know mrfast or BWA-short can do this for Illumina data, but is there any aligner that can do this that works on non-Illumina reads? My searches haven't turned up anything.

  • #2
    What type of non-Illumina reads? There are now a few categories out there. mrFAST should work for Ion Torrent. If you are using pacbio, the blasr parameter -bestn limits the number of hits you will get, up to 2^32 - 1.

    Originally posted by Rocketknight View Post
    Hi, I'm looking for an aligner that reports multiple/all hits rather than just a single one. I know mrfast or BWA-short can do this for Illumina data, but is there any aligner that can do this that works on non-Illumina reads? My searches haven't turned up anything.

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    • #3
      Good point. I'm interested in 454/Ion Torrent or PacBio reads, so thanks for the info! I didn't realize mrfast could handle Ion Torrent reads, they're pretty specific about calling it an Illumina read aligner on their site. I'll give it a try though!

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      • #4
        Have you tried using other aligners with parameters optimized for returning all hits like bowtie2 (-a), bwa mem (-a), novoalign (-r All) and tmap (mapall)? Thanks

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        • #5
          See Heng Li's announcement about BWA-MEM. http://www.biostars.org/p/67236/

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          • #6
            Oh god, I'm sorry. I kept looking through that manual and never saw that. Sorry!

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            • #7
              Glad it helped :-)

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