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Hi, I'm looking for an aligner that reports multiple/all hits rather than just a single one. I know mrfast or BWA-short can do this for Illumina data, but is there any aligner that can do this that works on non-Illumina reads? My searches haven't turned up anything.
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What type of non-Illumina reads? There are now a few categories out there. mrFAST should work for Ion Torrent. If you are using pacbio, the blasr parameter -bestn limits the number of hits you will get, up to 2^32 - 1.
Originally posted by Rocketknight View Post -
Good point. I'm interested in 454/Ion Torrent or PacBio reads, so thanks for the info! I didn't realize mrfast could handle Ion Torrent reads, they're pretty specific about calling it an Illumina read aligner on their site. I'll give it a try though!Comment
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Have you tried using other aligners with parameters optimized for returning all hits like bowtie2 (-a), bwa mem (-a), novoalign (-r All) and tmap (mapall)? ThanksComment
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Oh god, I'm sorry. I kept looking through that manual and never saw that. Sorry!Comment
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Glad it helped :-)Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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