Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Mapping reads against metabolical maps

    I need to make a functional analysis of a metagenome and therefore have to map a lot of color space SOLiD reads 75 bp each (sadly, assembly isn't any good) against metabolical pathways. Desired output will be something like a KEGG map, with every protein marked "Found in metagenome, reads #XXXXXXX, #YYYYYY" or "Not found".
    The first idea was a KEGG Mapper set of programs, but it requires KEGG orthologies as input, and getting those for every read without an account will require some creative hacking and violate their license. Are there any other big DBs for pathway maps, ideally linked to PFAM/Genbank/whatever for easy understanding?

  • #2
    I think I found what I was looking for, posting here in case someone will find it useful. Brenda Enzymes DB allows to get lots of enzyme information via SOAP interface, including accession codes for organisms. And further mapping will be straightforward.

    Comment


    • #3
      That's good. Thanks because I had the same problem

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Understanding Genetic Influence on Infectious Disease
        by seqadmin




        During the COVID-19 pandemic, scientists observed that while some individuals experienced severe illness when infected with SARS-CoV-2, others were barely affected. These disparities left researchers and clinicians wondering what causes the wide variations in response to viral infections and what role genetics plays.

        Jean-Laurent Casanova, M.D., Ph.D., Professor at Rockefeller University, is a leading expert in this crossover between genetics and infectious...
        09-09-2024, 10:59 AM
      • seqadmin
        Addressing Off-Target Effects in CRISPR Technologies
        by seqadmin






        The first FDA-approved CRISPR-based therapy marked the transition of therapeutic gene editing from a dream to reality1. CRISPR technologies have streamlined gene editing, and CRISPR screens have become an important approach for identifying genes involved in disease processes2. This technique introduces targeted mutations across numerous genes, enabling large-scale identification of gene functions, interactions, and pathways3. Identifying the full range...
        08-27-2024, 04:44 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Today, 06:25 AM
      0 responses
      13 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, Yesterday, 01:02 PM
      0 responses
      12 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 09-18-2024, 06:39 AM
      0 responses
      14 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 09-11-2024, 02:44 PM
      0 responses
      14 views
      0 likes
      Last Post seqadmin  
      Working...
      X