Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • gmarco
    Member
    • Oct 2012
    • 36

    CISA assembly contig integrator error

    Hello,

    I'm trying to use CISA to merge and optimize two de novo bacterial assemblies.
    Process seems to work fine until the very last step. I'm getting this error:

    Code:
    Traceback (most recent call last):
      File "CISA.py", line 63, in <module>
        os.rename('Contigs_'+final+'.fa',outfile)
    OSError: [Errno 18] Invalid cross-device link
    I've chmoded all dirs to 755. And I'm the owner of all the dirs.

    Any idea how to troubleshot this?

    CISA website: http://sb.nhri.org.tw/CISA/en/CISA
  • gmarco
    Member
    • Oct 2012
    • 36

    #2
    Seem to be a directory permission issue. CISA doesn't like NFS mount directories..

    Comment

    • yifangt
      Member
      • Feb 2011
      • 61

      #3
      solution to OSError

      I met same problem. Did a little bit search and found out one solution can be:
      Code:
      import shutil
      then change this line (Line 64 at CISA.py )
      Code:
         os.rename('Contigs_'+final+'.fa',outfile)
      to
      Code:
      shutil.move('Contigs_'+final+'.fa',outfile)
      It worked for me.

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
        by SEQadmin2



        Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
        ...
        07-09-2026, 11:10 AM
      • SEQadmin2
        Cancer Drug Resistance: The Lingering Barrier to Rising Survival
        by SEQadmin2



        Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

        There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
        07-08-2026, 05:17 AM
      • GATTACAT
        Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by GATTACAT
        Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
        07-01-2026, 11:43 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 07-13-2026, 10:26 AM
      0 responses
      20 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 07-09-2026, 10:04 AM
      0 responses
      30 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 07-08-2026, 10:08 AM
      0 responses
      20 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 07-07-2026, 11:05 AM
      0 responses
      34 views
      0 reactions
      Last Post SEQadmin2  
      Working...