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  • Rob2012
    Junior Member
    • Jan 2013
    • 6

    #1

    Parsing a GenBank file

    Hi,

    I would be happy if someone could help me get the following code to output the sequences (including the ids) of all records in the input file (http://biopython.org/DIST/docs/tutor...s_orchid.gbk):

    HTML Code:
     use Bio::SeqIO;
        use Bio::Seq;
        use Bio::DB::GenBank;
    
        $seq_obj = Bio::SeqIO->new(-file => "ls_orchid.gbk" , '-format' => 'genbank');
        $seqio_obj2 = Bio::SeqIO->new(-file => '>sequence.fasta', -format => 'genbank' );
    
        while ( my $seq = $seq_obj->next_seq() ) {
        print "Sequence ",$seq->id ($seq_obj)"\n";
        #print $seq_obj->seq,"\n";
        $seqio_obj2->write_seq($seq_obj);
        }
    I would like to see an output consisting of purely sequences (preferably with headers) as follows:
    HTML Code:
    seq1:
        ATTCGCTGCATGACAT..........
        Seq2:
        ACTGCGATGGATGGAT..

    Thank you
  • rhinoceros
    Senior Member
    • Apr 2013
    • 372

    #2
    You could try biopieces read_genbank
    savetherhino.org

    Comment

    • dpryan
      Devon Ryan
      • Jul 2011
      • 3478

      #3
      For those getting here later via google, the solution was posted on biostars.

      Comment

      • Rob2012
        Junior Member
        • Jan 2013
        • 6

        #4
        Thanks Rhinoceros.

        Comment

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