Tweet
My understanding of read mapping is that your generated reads are aligned to a reference sequence. Is the reference sequence/genome necessarily of the same organism or can the same process be applied to closely related species as well?
-
-
You can map to a related species, but most mapping software is designed for mapping to the same organism. Your mapping proportion will drop, and you'll probably miss out on capturing sequence where there has been a substantial change between the species. I have done mapping for environmental samples where I know the general species, but not the specific strain, of a microorganism.
FWIW, Bowtie2 has a "local align mode" that should work a bit better for mapping to closely related species.
-
Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
-
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
Comment