Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • wangsen123jay
    Junior Member
    • Mar 2014
    • 3

    #1

    blastn m0 -> m8 bug

    Hi,all

    Does anyone meet this question.

    Blastn Error:

    Error :m=0, E-value = e-141,Gaps=3, However when m=8,E-value is 2e-141, Gaps=2

    Tool:Blast

    The version number:blast-2.2.26 /blastn

    Parameter:default

    Query :DNA sequence, 1477 bp, test.fa

    Database: Rv0002, 1209 bp, Rv0002.fa

    Output:

    m=0

    Score = 490 bits (247), Expect = e-141

    Identities = 263/266 (98%), Gaps = 3/266 (1%)

    Strand = Plus / Plus

    m=8

    test Rv0002 98.87 266 0 2 944 1206 178 443 2e-141 490

    Thanks very much!
  • maubp
    Peter (Biopython etc)
    • Jul 2009
    • 1544

    #2
    Have you tried the current release, BLAST+ 2.2.29, which may well have fixed this?

    Comment

    • lindenb
      Senior Member
      • Apr 2010
      • 143

      #3
      cross posted on http://www.biostars.org/p/94956

      Comment

      • wangsen123jay
        Junior Member
        • Mar 2014
        • 3

        #4
        Originally posted by maubp View Post
        Have you tried the current release, BLAST+ 2.2.29, which may well have fixed this?
        Thanks a lot ,it's OK,I think may they have the different algorithm.

        Comment

        • wangsen123jay
          Junior Member
          • Mar 2014
          • 3

          #5
          Thanks a lot ,it's OK,I think may they have the different algorithm.

          Comment

          Latest Articles

          Collapse

          • SEQadmin2
            16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
            by SEQadmin2





            NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
            10-30-2026, 06:46 AM
          • SEQadmin2
            Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
            by SEQadmin2



            Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

            Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
            ...
            10-14-2026, 09:55 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by SEQadmin2, 11-05-2026, 09:25 AM
          0 responses
          20 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 11-03-2026, 11:54 AM
          0 responses
          34 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 10-28-2026, 09:57 AM
          0 responses
          82 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 10-28-2026, 09:51 AM
          0 responses
          50 views
          0 reactions
          Last Post SEQadmin2  
          Working...